Urine samples are generally collected by the patients themselves. The laboratory employee in charge of the sampling is responsible for informing the patient of the necessary recommendations and labeling the samples.
The samples are ideally collected at the laboratory. The collection can otherwise be carried out at the patient’s home, while respecting the conditions noted on the information sheets and the transport time, or the storage conditions (4° C) of the sample.
For catheterized patients, the biologists and nurses are trained to obtain the sample.
6.1 Urine microscopy and culture exam
– No antibiotic treatment, or drug holiday, or after at least 48 hours of treatment discontinuation,
– collection of urine that has preferably been in the bladder for 3 to 4 hours,
– the time period between the sample collection and the transport to the laboratory should be no longer than one hour following micturition.
6.1.2 Collection technique
184.108.40.206 In adults
-Label the bottle.
– Clean the urinary meatus with a mild antiseptic solution (Dakin’s solution or benzalkonium chloride on a single swab), then using a sterile compress soaked with saline or sterile distilled water.
– Discard the first stream.
– Open the bottle.
– Collect the urine mid-stream.
– Close the bottle immediately to avoid contaminants.
– Give the sample to the laboratory employee in charge of the sampling who will transport it to the laboratory.
NB: in patients with urinary catheters inserted less than 3 hours earlier, the sample will be taken from the tube after clamping it for several minutes and disconnecting the bag.
220.127.116.11 In infants
– Clean the genitals using Dakin’s solution or benzalkonium chloride. If it is an uncircumcised boy, retract the glans before cleaning it.
– Place a urine bag; change the bag every 30 minutes until urine is excreted.
– Fold the bag and place it in a sterile 150 ml jar.
– Label the sample (last name, first name, patient number).
– Take to the laboratory with the bench worksheet.
6.2 Urine chemistry
– No menstrual period for women.
– Fasting for the glucose measurement.
– For qualitative assays (investigations for albumin, sugar, ketone bodies, etc.), collect micturition in a sterile, 60-ml urine jar.
– For quantitative assays, use a 2-liter bottle and provide the patient with an information sheet.
NB: If the bottle is full in less than 24 hours, the patient can continue in another clean bottle and take them both to the laboratory.