Skin and appendage samples

Prior to the sampling

The sampling should be done in the laboratory before any specific treatment, whether local or systemic.

If the patient is receiving treatment, treatment should be interrupted for:

– 8 days for skin or hair lesions,

– 1 month for nails,

– no nail polish,

– wash the hair 3 days before the sampling and avoid greasy products.

The patient history is important: (information sheet to be filled in)

– ethnic origin,

– lifestyle (housing, profession, leisure activities, pets),

– risk factors (diabetes, immunosuppression, drug treatment).

7.1.1 Skin lesion samples 

– Identify the damaged area.

– Scrape the skin using a curette, a lancet or a scalpel blade.

– Take a sample of the skin flakes around the lesion.

– Place them in a small sterile glass Petri dish.

– If the lesion is suppurated, collect the pus with a swab that has been moistened with sterile normal saline.

– Take to the laboratory after labeling.

7.1.2 Follicle sampling and sycosis

 – The hair and down are to be sampled with tweezers.

– Apply a swab moistened with sterile distilled water to lesions with exudate.


7.1.3 Sampling of ringworm from the scalp  

– Identify the damaged area.

– Examine the scalp with a Wood’s lamp green fluorescence.

– Using a lancet or a scalpel, scrape the skin: sampling of the periphery.

– Using a curette and a tweezers, take specimens of the skin flakes, broken hair and scabs in the proximal area (at the border of the healthy skin and damaged skin). A minimum of 10 hairs with their root are necessary.

– On patches of alopecia, apply a moistened swab to suppurated lesions.

– Label and take to the laboratory.

7.1.4 Nail sample (Onychitis)

– Cut and discard the edge of the nail with nail clippers or scissors.

– Using a nail clipper, a curette or a lancet, take a sample of the pathologic nail area from the border between the healthy part and the damaged part (where the dermatophyte is most active).

– Scrape the nail bed and place all the results in a sterile glass Petri dish.

– Label and take to the laboratory.

7.1.5 Intertrigo sample

 – Scrape the edge of the lesions with a curette.

– Swab the edges of the lesion.

– Label and take to the laboratory.

Introduction Sample manuel

List of laboratory tests

Consent forms


Urines sample collection procedures

Urgent sample

Skin and appendage samples

Subcontracted tests

Manegement of sempling incident

Manegemtn of blood exposure

Medical Monitoring form Medical

Sample sheet

Sample transport

Waste disposal

Additional tests

Sample storage