Sims-Hühner or postcoital test (PCT)

7.1.1 Conditions

 – Middle of the menstrual cycle (12th or 13th day of the cycle),

– sexual abstinence for both partners for a minimum of 3 days and maximum of 5 days,

– sexual intercourse performed preferably around midnight; stay laying down and come to the laboratory, without washing the genitals, around 9 o’clock in the morning,

– take the sample between 8 and 12 hours after intercourse.

7.1.2  Sampling technique

 – Place the patient in the lithotomy position;

– Insert a sterile speculum in the vagina so that the cervix is well visualized;

– If there is abundant leaking, wipe the vagina with sterile gauze.

– Record the degree of cervical opening (punctiform, open or wide-open).

– Record the quantity of mucus (minimal, droplets or flowing).

– Use the Aspiglaire (mucus sampling syringe).

– Insert the tip of the device into the cervix about 1 cm;

– Gently aspirate the mucus (avoid contaminating the endocervical mucus with the vaginal mucus).

– Use another Aspiglaire and take a sample from the vaginal fornix.

– Take the samples and lab sheet to the bacteriology laboratory and place the samples in the 37° C incubator.

Introduction Sample manuel

List of laboratory tests

Consent forms


Urines sample collection procedures

Urgent sample

Skin and appendage samples

Subcontracted tests

Manegement of sempling incident

Manegemtn of blood exposure

Medical Monitoring form Medical

Sample sheet

Sample transport

Waste disposal

Additional tests

Sample storage