Pap smear

7.1.1 Conditions

Not during the menstrual period.

Monolayer technique  Presentation of the monolayer cytology technique

The monolayer smear technique is a technological innovation that improves the quality of the smear for cervical cancer screening.

This technique provides:

– sampling ease for the practitioner,

– sampling done in a single movement using a brush, which is then transferred to a bottle with cell preservative,

– a collection of all the cellular material obtained, which  results in a better sample,

– a significant decrease in the number of uninterpretable smears due to poor fixation, the presence of blood, excessive white blood cell, superimposed cells, stretching or crushing of cells,

– easy reading for the cytopathologist,

– the possibility of performing other tests from the same specimen sample (viral typing, etc.).  Sampling protocol (for thin layer smear)

This technique is used for monolayer smears:

 – Collect a cell specimen sample from the cervix by inserting the central part of the brush into the endocervical canal so that the lateral bristles are in contact with the exocervix; turn the brush (at least 2 turns).

– Place the brush in the bottle containing the preservative solution.

– Close the cap tightly.

– Place the label from the sampling sheet on the bottle and fill in the “Cytology Test” form.

– Take everything to the laboratory.

Introduction Sample manuel

List of laboratory tests

Consent forms


Urines sample collection procedures

Urgent sample

Skin and appendage samples

Subcontracted tests

Manegement of sempling incident

Manegemtn of blood exposure

Medical Monitoring form Medical

Sample sheet

Sample transport

Waste disposal

Additional tests

Sample storage