– Middle of the menstrual cycle (12th or 13th day of the cycle),
– sexual abstinence for both partners for a minimum of 3 days and maximum of 5 days,
– sexual intercourse performed preferably around midnight; stay laying down and come to the laboratory, without washing the genitals, around 9 o’clock in the morning,
– take the sample between 8 and 12 hours after intercourse.
7.1.2 Sampling technique
– Place the patient in the lithotomy position;
– Insert a sterile speculum in the vagina so that the cervix is well visualized;
– If there is abundant leaking, wipe the vagina with sterile gauze.
– Record the degree of cervical opening (punctiform, open or wide-open).
– Record the quantity of mucus (minimal, droplets or flowing).
– Use the Aspiglaire (mucus sampling syringe).
– Insert the tip of the device into the cervix about 1 cm;
– Gently aspirate the mucus (avoid contaminating the endocervical mucus with the vaginal mucus).
– Use another Aspiglaire and take a sample from the vaginal fornix.
– Take the samples and lab sheet to the bacteriology laboratory and place the samples in the 37° C incubator.