Glucose tolerance tests

Inform the patient on the length of time to be spent in the laboratory for the performance of the dynamic tests

 5.1.1 Fasting and postprandial blood glucose

The sample for fasting blood glucose should be taken after a fast of 8 to 12 hours.

The sample for postprandial blood glucose is to be done 2 hours after the start of the meal (breakfast or lunch). The patient should continue his/her regular routine and should take his/her medicines if currently on treatment.

 5.1.2 Oral glucose tolerance test

5.1.2.1 Conditions:

– This exam is done from Monday to Friday at the laboratory, only in the morning and can take 3 to 4 hours.

– Patient fasting for 8 to 12 hours, physical rest during the test (lying or sitting).

– Do not eat, drink or smoke during the test.

– Do not perform the test in patients over the age of 70 years.

– Do not perform in diabetic patients.

– Do not perform if the following medications are being used:

  • Antihyperglycemic agent: Corticosteroids, estrogens, thiazide diuretics,
  • Hypoglycemic agents: aspirin, quinine, perhexiline, dysopyramide.

5.1.2.2 Protocol:

– Place a catheter mounted on a syringe rinsed with a heparin solution in normal saline.

– Take a blood sample from the fasting patient (time T0).

– Take to the laboratory for the fasting blood glucose assay.

– If the fasting blood glucose value is less than 1.26 g/l, have the patient slowly drink (5 minutes) “GLUCO 75” or 250 ml of 30% hypertonic glucose solution (75 g) in adults, or 5.8 ml of hypertonic solution per kilo of weight (1.75 g/kg) in children, not exceeding 75 g.

– Collect the samples in tubes without additives at the following times:

  • T0 + 30 min
  • T0 + 60 min
  • T0 + 90 min
  • T0 + 120 min
  • T0 + 150 min
  • T0 + 180 min

NB: Bring the properly labeled blood samples to the laboratory as they are collected. Record any signs of hypoglycemia (vertigo, sweating). If the patient faints, take a blood sample, record the time, stop the test and give a sweetened juice.

If the fasting blood glucose value is higher than 1.26 g/l, notify the doctor before continuing the             test.

5.1.3 O’Sullivan test

5.1.3.1 Aim of the test

Provides early screening for gestational diabetes. It is done in the 6th month of pregnancy in women with a risk factor for diabetes. It is a simplified hyperglycemic test.

5.1.3.2  Conditions

Patient fasting

5.1.3.3  Protocol

O’Sullivan test with 50 grams of glucose

– Take a blood sample from the fasting patient (time T0).

– Have the patient urinate to check the sugar level.

– Have the patient drink a bottle of “GLUCO 50” or 166 ml of 30% glucose solution (50 g of glucose) in less than 5 minutes.

– Take a sample at T0 + 60 minutes.

O’Sullivan test with 75 grams of glucose

– Take a blood sample from the fasting patient (time T0).

– Have the patient urinate to check the sugar level.

– Have the patient drink a bottle of “GLUCO 75” or 250 ml of 30% glucose solution (75 g of glucose) in less than 5 minutes.

– Take a sample at T0 + 120 minutes.

Introduction Sample manuel

List of laboratory tests

Consent forms

SAMPLE IDENTIFICATION

Urines sample collection procedures

Urgent sample

Skin and appendage samples

Subcontracted tests

Manegement of sempling incident

Manegemtn of blood exposure

Medical Monitoring form Medical

Sample sheet

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Waste disposal

Additional tests

Sample storage